Plant-parasitic nematodes (PPNs) pose a significant threat to crop production in British Columbia, particularly for high-value horticultural crops. In perennial fruit systems, PPN populations can accumulate undetected over several years, establishing themselves in the root zones of long-lived crops. This buildup can lead to substantial yield losses, especially when fields are replanted without prior assessment or treatment. 

Early detection and accurate quantification of PPNs in soil prior to replanting are critical for effective crop management. Identifying whether PPNs are present at economically damaging levels enables growers to make informed decisions about implementing control measures, such as pre-plant soil fumigation or post-plant nematicide applications. 

Quantitative real-time PCR (qPCR) is a powerful molecular diagnostic tool widely used for detecting and quantifying plant pathogens, including nematodes. This project builds on previous research efforts to validate and refine qPCR tests specifically for the detection of economically important PPN species in British Columbia. 

The study will validate eight distinct qPCR tests to identify and quantify key PPN species across soil samples collected from berry farms, tree fruit orchards and vineyards in the Okanagan and Fraser Valleys. These regions represent major horticultural production zones in the province. 

By supporting the development of robust diagnostic tools and expanding access to reliable testing services, this initiative will empower producers to better manage PPN populations. Ultimately, this will contribute to improved crop productivity, reduced economic losses and enhanced sustainability within B.C.’s horticultural sector.