The realities of global warming and the volatile oil market have triggered a movement to replace part of the fossil fuel reliance with renewable fuels. A solution was proposed to use cellulose as feedstock for ethanol production. There is a real need to improve the efficiency of the procedure to isolate cellulose, primarily by decreasing the amount of tree lignin for this to become a viable solution. Whilst fast-growing hybrid poplar trees are likely to become the most important and universal cellulosic feedstock in Canada, there was not yet a single poplar variety developed for this purpose. This research identified genetic markers guiding the development of genomics-based selection technology to rapidly generate hybrid poplar varieties that are more suitable as feedstock for ethanol production.