Next-generation sequencing has made it feasible to sequence cancer genomes to determine the underlying genetic causes of the disease.
The objective of this project was to establish tools for the rapid generation of mouse models carrying mutations through the following:
1. Development of a model system by using human cell lines that were altered to harbor specific mutations.
2. Establishment of a genome editing system to rapidly generate mouse lines with lymphocyte inducible expression of driver mutations
Once established, these techniques will be suitable for modelling non-lymphoma cancer mutations, as well as mutations identified in non-cancerous diseases. Researchers in a broad spectrum of fields will be able to take advantage of the tools generated in this project. Moreover, the mouse models generated will provide a valuable resource for further study of driver mutation functions.