Project Search

sector_ico_Health_trans Human Health

Functional Characterization of the Transcriptional Network Driving Mammalian Brain Development

  • Project Leaders: Daniel Goldowitz
  • Institutions: University of British Columbia (UBC)
  • Budget: $330000
  • Program/Competition: Strategic Opportunities Fund
  • Genome Centre(s): Genome British Columbia
  • Status: Closed

Normal brain development is a very complex process that involves many discrete, sequential events. When the order or timing of these brain development events is not perfect, severe problems with cognitive and motor function can result, as well as diseases such as schizophrenia, Fragile X mental retardation and autism spectrum disorder.

This project aimed to catalog the regulation of gene expression on the developing embryonic mouse cerebellum using CAGE (Cap Analysis of Gene Expression) technology developed at the RIKEN Institute and Next Generation Sequencing through a successful collaboration with the RIKEN Institute in Japan.

The data generated by this project is the first of its kind focusing on specifically cataloguing small RNAs during cerebellar development. All the deepCAGE and MARA data regarding the transcriptional network of cerebellar development are publicly available through the RIKEN and GRiTS websites. All CAGE data is mapped to the mouse genome and is available on the web to download by FANTOM5 collaborators. The genome track data with CAGE tag counts are viewable through RIKEN’s in-house developed ZENBU browser. This will promote and expand knowledge translation to the broader scientific community through the use of new tools, technologies and methodologies.